DNA

Part:BBa_K4342019:Design

Designed by: Keaton Brown   Group: iGEM22_Austin_UTexas   (2022-10-11)


ACIAD2049 Integration Cassette


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1040
    Illegal EcoRI site found at 3585
    Illegal XbaI site found at 180
    Illegal XbaI site found at 2000
    Illegal XbaI site found at 3505
    Illegal SpeI site found at 3183
    Illegal PstI site found at 645
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1040
    Illegal EcoRI site found at 3585
    Illegal NheI site found at 4124
    Illegal SpeI site found at 3183
    Illegal PstI site found at 645
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1040
    Illegal EcoRI site found at 3585
    Illegal BglII site found at 3343
    Illegal BglII site found at 3841
    Illegal BamHI site found at 1994
    Illegal XhoI site found at 2156
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1040
    Illegal EcoRI site found at 3585
    Illegal XbaI site found at 180
    Illegal XbaI site found at 2000
    Illegal XbaI site found at 3505
    Illegal SpeI site found at 3183
    Illegal PstI site found at 645
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1040
    Illegal EcoRI site found at 3585
    Illegal XbaI site found at 180
    Illegal XbaI site found at 2000
    Illegal XbaI site found at 3505
    Illegal SpeI site found at 3183
    Illegal PstI site found at 645
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

References